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Purpose: Identify GUS-positive E
It has also been used as a selection tool for mutant colonies
though optimized for DNA
and Golden Gate assembly systems used in iGEM projects
Camptothecin (Mappia foetida) Size:250 mg Purpose: Identify GUS-positive ECamptothecin is an alkaloid compound used as an anti cancer agent. It is a topoisomerase I inhibitor in DNA synthesis. Camptothecin has been shown to bind and stabilize a topoisomerase I DNA complex in vitro, preventing the enzyme from reannealing DNA strands. This inhibition specifically effects the S phase of cell development and ultimately results in apoptosis due to damaged DNA. Camptothecin is found in Mappia foetida (Nothapodytes foetida), a
