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For Real-Time PCR amplification, the Maxima SYBR Green qPCR Master Mix was used, with primers designed for the target genes StAR (Steroidogenic Acute Regulatory Protein), 3-HSD (3-Hydroxysteroid Dehydrogenase), Cyp17a (Cytochrome P450 17A1), Aromatase and GAPDH as an internal control

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