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Animals and housing Following laboratory animal welfare (3Rs: Replacement, Reduction, and Refinement) best practice [31], we acquired 13 adult female mice (C57BL/6 background) from another lab which were excess to experimental requirements, experimentally nave, and slated for euthanasia

243 Microglial activation and senescence are key features of AD and are linked to abnormal glycolysis and inflammation

In summary, as an important factor of aging, oxidative stress can also mediate telomere damage in the ovaries and cause ovarian aging (Figure 1)

If the constipation concerns you, raise it before continuing the protocol