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Chronic inflammation marked by elevated IL-6 disrupts systemic metabolism, activating other pro-inflammatory cytokines like TNF- and IL-1, creating a feedback loop that intensifies inflammation and insulin resistance [11,12]

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2.4.3 Analysis of liver antioxidant indexes Seven bullfrogs were taken from each of the NC, FI and GSH groups for liver tissue extraction, and the livers were quickly put into liquid nitrogen after removal, and then stored in a -80C refrigerator for the detection of liver antioxidant indexes

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