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The samples were then centrifuged at 18 000 g at 4 C for 15 min, and 20 l of the resulting supernatants was injected into the HPLC column for metabolite quantification, while the pellet was used for protein analysis using the BCA protein assay

Use a fresh, sterile syringe each time you pull liquid

Mass spectrometry analysis was performed on a Q-Exactive HF MS (Thermo Fisher Scientific, Waltham, MA, United States) using a data-dependent acquisition model

Saxenda is contraindicated in patients with a family history of medullary thyroid cancer (MTC) or in patients with Multiple Endocrine Neoplasia syndrome type 2 (MEN 2)