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On the 6th day, mice were given 0.15% saccharine instead of water for 0.5 h, and then injected with saline (10 ml kg 1 , p.o.), d-allulose (1 and 3 g kg 1 , 10 ml kg 1 , p.o.), or lithium chloride (LiCl, 3 nmol, 20 ml kg 1 , i.p.)

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The 0.9% benzyl alcohol helps inhibit bacterial growth between uses when proper technique is employed