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For the chromatographic metabolite separation, the Vanquish UPLC systems were coupled to a Q Exactive HF (QE-HF) mass spectrometer equipped with HESI (Thermo Fisher Scientific, Waltham, MA)

These require larger injection volumes to achieve the same dose, which some patients prefer (easier to measure precisely with an insulin syringe) and others dislike (more fluid volume under the skin)

Unlike regular anti-inflammatory drugs, BPC-157 works in multiple ways without known harmful side effects

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