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Glutathione, a tripeptide composed of -glutamic acid, cysteine and glycine is quantitatively the most important low-molecular-weight (non-protein) thiol in tissues[6, 9, 10]

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Immunofluorescence U251 or U3085 cells were fixed and immunostained to allow for automated quantification of the percentage of cells with SGs and the average number of SGs per cell (in those cells with SGs) based on correlative TIAR and G3BP2 immunostaining as previously described [18]

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