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All mice received 2 days of 10 mL/kg body weight sterile saline subcutaneous injections before beginning the treatment phase to allow for acclimation to the handling and stress associated with injections

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Situations or conditions that can cause vitamin B12 deficiency include: You are more likely to develop vitamin B12 deficiency if you have one or more of the following risk factors: Left untreated, vitamin B12 deficiency can cause lasting side effects that affect your nervous system and brain

Vitamins tend to work in a system of use and replenish. Vitamin B12 travels through the bloodstream and is eventually expelled through urine