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Continuous, non-pulsatile GH elevation is actually a problem: sustained receptor activation leads to desensitization and blunted response
Another even simpler way to think of oxidative stress is to use the definition from the Cleveland Clinic*: Oxidative stress is an imbalance between two different types of molecules in your body: free radicals and antioxidants
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Then, cells were collected and fluorescence ( ex = 405 and 488 nm, em = 510 nm) determined by flow cytometry as described above 35
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