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In contrast, DAG may activate protein kinase C (PKC), potentially heightening the signaling pathway and possibly facilitating the secretion of growth hormone from pituitary cells

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Protein intake timing is critical because IGF-1 activates mTOR, the nutrient-sensing kinase that drives protein synthesis only when sufficient amino acids (particularly leucine at 2.53g per meal) are present to co-activate the pathway without adequate dietary protein, IGF-1 receptor activation occurs but downstream translation of mRNA into new muscle protein is blunted by lack of substrate

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