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Despite their promising effects and low cost, TCAs should be prescribed cautiously because of the side effects such as dry mouth, constipation, urinary retention, and orthostatic hypotension

In the measurements, basal fluorescence was first recorded for 30 s until the TRPA1 agonist (HY-100965, 100 M) was added at 37 C, and the following measurement ensued for 60 s

However, the medication still produces central nervous system effects through indirect pathways, including vagal signaling and action at circumventricular organs