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step-b) Fmoc-Thr(tBu)-Phe-Thr(tBu)-Ser(tBu)-Asp(OtBu)-Val-Ser(Oxa)-OH (fragment-2) was condensed with peptide obtained from step-a) in presence of a coupling agent to obtain Fmoc protected Thr(tBu)-Phe-Thr(tBu)-Ser(tBu)-Asp(OtBu)-Val-Ser(Oxa)-Ser(tBu)-Tyr(tBu)-Leu- Glu(OtBu)-Gly-Gln(Trt)-Ala-Ala-Lys(C18diacidmono-t-butyl-Y-Glu(AEEA-AEEA)-OtBu)-Glu (OtBu)-Phe-Ile-Ala-Trp(Boc)-Leu-Val-Arg(pbf)-Gly-Arg (pbf) Coupling agent used in the reaction is preferably EDC.HC1 and HOAt in DMF

Pickering G, Macian N, Papet I, et al

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