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doi: 10.3389/fmicb.2016.01925 Received 22 July 2016 Accepted 16 November 2016 Published 06 December 2016 Volume 7 - 2016 Edited by Miguel Cacho Teixeira, University of Lisbon, Portugal Reviewed by Hans Uwe Dahms, Kaohsiung Medical University, Taiwan

Consenting patients over 18 years that had been treated with rtPA for an ischemic stroke with preserved swallowing function and without a recent stroke (within 3 months) or previously known diabetes were eligible for participation

As reported for nitric oxide synthase whose K M value for arginine is much greater in cells than that in assay tubes (Wu and Meininger 2000), it is possible that the K M of carnosine synthetase for histidine in vivo is much greater than that (i.e., 16.8 M) reported for the purified enzyme under in vitro assay conditions, due to complex proteinprotein and enzymesubstrate interactions in vivo

Future challenges and therapeutic opportunities in type 2 diabetes: changing the paradigm of current therapy