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Each liver was homogenized in 3ml of 0.25 M sucrose per g of liver, and centrifuged at 3,000 and 10,000 rpm for 10 minutes respectively to precipitate cell debris

The determination of the optimal and appropriate duration of selenium supplementation for GD, as well as the proper selection of the most appropriate prebiotic compound and the required Prebiotic Index (PI), which could at best be obtained by matching certain prebiotics with certain probiotic species, optimized in each patient

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Moreover, the defective ATP production is easily compensated by the increased activity of enzymes in glucose utilization, providing the cell with alternative substrates but not increasing energy levels but rescuing the affected activities and pathways by stabilizing the mitochondrial membrane lipid components ( Abnormal lipid metabolism with oxidative stress, ferroptosis and T cell dysfunction Previous studies on a small number of patients have reported conflicting results regarding the association between vitiligo and dyslipidemia