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lst-1 and sygl-1 were identified in a small candidate RNAi screen of 15 genes that meet the following criteria(i) the gene contained a cluster of at least four LAG-1 binding sites [23] and (ii) its mRNA was a target of FBF-1 regulation based on FBF-1 immunoprecipitation followed by microarray analysis [20,28]
Compound 28 demonstrated exceptional in vivo potency, with an IC 50 of 4.2 nM, alongside a favorable safety profile, making it a promising candidate for further preclinical research
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