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In atherosclerosis, proteolysis-resistant GLP-1RA NPs not only visualize plaques via MRI but also suppress smooth muscle proliferation, enabling simultaneous efficacy assessment and disease modification [197]

An equal volume of 2 urea sample buffer (150 mM Tris pH 6.8, 6 M Urea, 6% SDS, 40% glycerol, 100 mM DTT, 0.1% bromophenol blue) was added to the lysates and heated at 65C for 5 minutes