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Naturally present in plasma, saliva, and urine, its levels decline with age, correlating with slower healing and visible tissue degeneration

Research interest nonetheless expanded considerably through the 1980s and 1990s, as investigators began exploring DSIP's broader neuroendocrine roles, including its interactions with the HPA axis, circadian timing systems, and stress-response pathways, establishing the compound as a multifunctional regulatory neuropeptide whose biological significance extends well beyond its original sleep-focused characterization

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VHMs showed numerous degradative autophagic vacuoles (black arrows) containing melanin or melanosomes (Fig