Subsequently, 100 g of protein from each sample was taken, the volume was adjusted to 100 L, and the mixture was incubated with 2.5 L of 400 mM DTT for 30 min at 37 C, followed by 5 L of 800 mM IAA for 15 min at room temperature in the dark
Therefore, some clients may need more frequent injections due to increased vitamin B12 utilization or loss
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Thanks also to Christian for the MD simulations, and to Amedeo, Jason, Winston, and Jorge for their valuable advice, support, and help along the way
