To evaluate the effects of compound on GPX4 activity, compound was first pre-incubated with purified GPX4 in the assay buffer [Tris-HCl (100 mM, pH 7.4), EDTA (5 mM), Triton X-100 (0.1% v/v), NADPH (0.2 mM), glutathione (3 mM), and glutathione reductase (1 unit)] for 5 min at 37C
After the percentage record of linear motile spermatozoa at 200 magnification under a phase contrast microscope in three different microscopic fields for each sample, the final motility ratio of frozen-thawed sperm motility in each group was calculated based on the mean of three successive estimations (27, 55, 57)
Frequency: one subcutaneous (or intramuscular) injection each day
While both peptides are investigated for their roles in tissue regeneration, they operate via distinct biological pathways