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The proposed protocol involves in vitro and in vivo studies, including cell-based assays, animal models, and biochemical analyses

A 1987 study on mice determined oral glutathione to be nontoxic,27 and a 2017 toxicity study (also on mice) found that glutathione's LD (the amount of a substance needed to kill 50% of all test animals in one dose) was 5g/kg.6 An equivalent toxic dose for an 80kg human would be 32.5g, which is more than 32 times the highest amounts used in clinical studies