For patients in South Florida, the heat outside the refrigerator is an especially important factor: a peptide vial left on a counter in a Doral kitchen during summer is exposed to ambient temperatures that can reach the mid-80s Fahrenheit or higher, which meaningfully accelerates degradation for any reconstituted solution left out during injection preparation
ECL3 and the top of TM7 are tilted slightly away from the peptide, adopting a marginally more open TM bundle than observed in AMYRs
Meanwhile, the body temperature of mice from different groups did not vary significantly from each other, and weekly food consumption displays a large fluctuation between groups, but the trend is consistent between groups (Figures 3C,D)
Our quality assurance program includes High-Performance Liquid Chromatography (HPLC) to confirm peptide identity and purity at 99%, and Mass Spectrometry (MS) to verify molecular weight matches the expected 403.93 Da, confirming correct amino acid sequence and copper complexation
