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The construct was expressed in Spodoptera frugiperda ( Sf 9) insect cells (Invitrogen) with Bac-to-Bac Baculovirus system (Invitrogen) and cells were infected at a density of 2 10 6 cells per mL and collected 48 h after infection

Everyones weight-loss timeline is different, but most people start experiencing appetite changes during their first week on a GLP-1, with noticeable weight loss occurring after 48 weeks

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