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2 Materials and methods 2.1 Drugs and chemicals To carry out this study, the drugs purchased and used were glycitin and PTZ (Sigma company, United States), phenobarbital ((PB) manufactured by Chemidarou Pharmaceutical company, Iran), ketamine and xylazine (Alfasan Company, Netherlands)

Common protocols include: Loading phase: 2-3 injections per week for 2-4 weeks Maintenance phase: 1 injection per week, or every 10-14 days Combined protocol: Monthly NAD+ IV infusion plus weekly injections Dosing should be individualized

Avoid injecting close to visible veins or previous injection sites to minimize discomfort or skin irritation

Monique Ramsey (23:22): And what are the procedures that people are most curious about after they lose weight