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Transformed cells were cultured in LB medium at 37 C until reaching an OD 600 of 0.50.6, then induced with 0.5 mM IPTG at 16 C for 1618 h

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Sitagliptin In patients with type 2 diabetes mellitus, administration of sitagliptin led to inhibition of DPP-4 enzyme activity for a 24-hour period

This inconsistency can affect both efficacy and side effects, as the body repeatedly adjusts to changing medication levels