Weight loss stabilizes after 12 to 18 months, but insulin sensitivity, blood sugar control, blood pressure, and lipid levels remain improved
After blocking with 10% milk for 1 h, the membrane was incubated with primary antibodies, including TH (T2928, SigmaAldrich, 1:2000), cleaved-caspase-3 (9661 s, Cell Signaling Technology, MA, USA, 1:1000), -actin (A5060, SigmaAldrich, 1:5000), LC3 (L7543, SigmaAldrich, 1:1000), cleaved-caspase-9 (9507 s, Cell Signaling Technology, 1:1000), p62 (ab56416, Abcam, Cambridge, MA, USA 1:1000), Drp-1 (ab184247, Abcam, 1:1000), Opa-1 (ab157457, Abcam, 1:1000), cytochrome c (ab133504, Abcam, 1:5000), AIF (ab1998, Abcam, 1:1000), VDAC (4661 s, Cell Signaling Technology, 1:1000), mTOR (4517 s, Cell Signaling Technology, 1:1000), p-mTOR (5536 s, Cell Signaling Technology, 1:1000), following by incubated with secondary antibody, and protein bands were visualized by enhanced chemiluminescence
Generally no they engage different mechanisms
WB protein band intensity was quantified using Image J software
