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The animals were then perfused with cold saline to free them of residual blood, after which liver and other tissues were collected immediately, weighed, quickly diced, and frozen in liquid nitrogen at 80 C

This mechanism is proposed as the results of the reinterpretation of toxicological and biological experiments in the light of our physico-chemical measurements of the fundamental properties of the involved molecules, the conjugates of the thiol-binding metals with the soluble and protein thiolome, and of the corresponding disulphides