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Rarely, allergic reactions can occur, presenting as hives, difficulty breathing, or swelling of the face, lips, tongue, or throat

The human CRC cell lines (SW480, SW620, HCT116, and LOVO) were cultured in the Roswell Park Memorial Institute medium (RPMI) 1640 (high glucose) (Gibco Laboratories, Gaithersburg, USA) supplemented with 10% FBS (Gibco Laboratories)