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To generate the GFP-fusion of Go-Nt 7 and Go-Nt 31 , the fragments were PCR-amplified (primers listed in Supplementary Data 3) from Go-GFP, products were cut with KpnI/AgeI and cloned in frame into the same sites of pEGFP-N1 (Clontech)
doi: 10.3389/fphar.2022.931746 Received 29 April 2022 Accepted 30 August 2022 Published 21 September 2022 Volume 13 - 2022 Edited by Paul Cockwell, University Hospitals Birmingham NHS Foundation Trust, United Kingdom Reviewed by Anil Bhanudas Gaikwad, Birla Institute of Technology and Science, India Eleanor DeLand Lederer, University of Texas Southwestern Medical Center, United States Updates Copyright 2022 Yu, Li, Sha, Kong, Ye and Liu
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