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We hypothesized that the hepatic metabolome would be similarly characterized by increased alternative energy pathway utilization, altered redox balance and glutathione depletion, and reduced levels of ATP

It incorporates key modifications, including 14E/17R mutations to stabilize an -helical segment via a salt bridge, multiple proline substitutions (25P, 28P, 29P) to reduce -sheet formation, and N-terminal C20 fatty diacid lipidation for reversible albumin binding (Kruse et al