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It were analyzed by sampling the right lumbar level 4,5,6 dorsal root ganglion (RL4,5,6-DRG) and lumbar level 5 spinal cord (L5-SC) from 3 rats of control, morphine, 3 BPC-157 groups at 5 (representative value of phase 1) and 35 min (representative value of phase 2) after the formalin test

Cells were mixed with transfection reagents and plated at 13,000 cells per well into 96-well Greiner CELLSTAR white-walled plates (Sigma-Aldrich), and assays were performed 48 h later

Interestingly, neutrophils of the horse lack defensins that depict mostly the main peptide type of these cells in other species

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