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However, this could reflect differences in the TMD of GLP-1R and GCGR and their intrinsic ability to activate G protein in the absence of agonist, independent of the ECD

Methods Reagents and chemicals [ 15 N, 13 C 5 ]valine, [ 15 N, 13 C 4 ]threonine, [ 13 C 3 ]propionate, D9 Carnitine, [2,2,2- 2 H 3 -1,2- 13 C 2 ]acetate, 20 M [2,2,3,3,3- 2 H 5 ]propionate, [2,2,3,3,4,4,4- 2 H 7 ]butyrate, [2,2,3,3,4,4,5,5,5- 2 H 9 ]pentanoate, [6,6- 2 H 2 ]glucose (D2 glucose), and [2,2,3,3,4,4,5,5,6,6- 2 H 11 ]hexanoate were from Cambridge Isotope Laboratories (Tewksbury, MA)

Agonists differ in affinity, selectivity, maximal response, and signalling bias

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