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Sacpic staining results also showed that the proportion of primary active hair follicles has no significant change, while the proportion of secondary active hair follicles in the KO group was significantly higher than that in the control group

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Concurrently, GO analysis of biological processes showed significant enrichment of the canonical Wnt signaling pathway, non-canonical Wnt signaling pathway, BMP signaling pathway, hair follicle development, hair cycle, cell migration, and positive regulation of stem cell proliferation (Figure 4B)