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In FD patients with unexplained loss of appetite, gastric phase III contractions are suppressed, suggesting low plasma levels, but these were not measured in this study (99)

EV4E) and RT-qPCR analyses (Fig

All cell lines were treated with a fixed dose of MTX (25 nM) for 24 hours, as this dose was representative of MTXs average IC50 at 96 hours across all cell lines (ie, 18.4 nM)

Moreover, gut microbiota dysbiosis, resulting from intestinal I/R injury, increases intestinal permeability, allowing bacteria and their products (e.g., lipopolysaccharide, LPS) to translocate into the circulation, activating systemic inflammation and disrupting the BBB