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GPX activity is calculated as NADPH oxidized/min/mg protein = [(change in absorbance at 340 nm/min for the sample test change in absorbance at 340 nm/min for the blank) final volume]/[0.00622 concentration of the sample in mg/mL protein sample volume], where 0.00622 = micromolar extinction coefficient of NADPH at 340 nm for the 1 cm path length of the quartz cuvette

2) Investigate possible correlations of the severity of autism with urinary excretion of toxic metals and initial level of glutathione

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This is due to that BBR relieves obesity mainly by inhibiting mitochondrial complex I in the gut and liver of mice, and this process is not affected by gut microbiota