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The supernatant was further centrifuged at 12,000 rpm for 3 min at 4 C, and 50 L of the resulting supernatant was prepared for liquid chromatography-tandem mass spectrometry (LC-MS) analysis

Supporting these findings, a recent PET (positron emission tomography) imaging study by (Kato et al., 2023) demonstrated a significant reduction in Complex I availability in the anterior cingulate cortex of ASD individuals, reinforcing the hypothesis that compromised mitochondrial respiration plays a key role in ASD pathophysiology

Diet, exercise, and overall lifestyle remain the most important factors for weight management