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In the treatment of liver cancer, Erastin can trigger de-O-GlcNAcylation of TFRC at serine 687 (Ser687), thereby reducing the binding of the ubiquitin E3 ligase membrane-associated RING-CH8 (MARCH8) and decreasing polyubiquitination on lysine 665 (Lys665), which enhances the stability of TFRC that favors the accumulation of labile iron and promotes the occurrence of ferroptosis[63]

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On the other hand, the non-tumorigenic, spontaneously immortalized keratinocyte cell line - HaCaT cells can provide almost unlimited supply of identical cells, ensuring high intra- and inter-laboratory reproducibility

Health Press, 1996