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In HF, the failing heart shifts its fuel source preference from fatty acids to glucose, whereas AD is characterized by reduced neuronal glucose uptake and OXPHOS activity

The resultant single cells were stained with antibodies purchased from Biolegend (USA): PE/Cy7 anti-mouse CD45 (1:400, #103113), FITC anti-mouse CD3 (1:400, #100203), APC anti-mouse CD4 (1:400, #100411), PE anti-mouse CD8 (1:400, #100707), APC anti-mouse IFN-(1:100, #505809)

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Color inspection is a necessary first step but should be combined with checking the BUD, ensuring proper storage conditions have been maintained, and obtaining a COA from your pharmacy