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A Initial injection Conditions Stationary phase: Kromasil 100-10-C4 Mobile phase solvent: acetonitrile Mobile phase salt: ammonium acetate Pool analysis, 95% purity Conditions Phase: Kromasil 100-3.5-C4 Eluent: acetonitrile / water Eluent salt: trifluoroacetic acid B After 30 sequential prep injections Conditions Stationary phase: Kromasil 100-10-C4 Mobile phase solvent: acetonitrile Mobile phase salt: ammonium acetate Pool analysis, 77% purity Conditions Phase: Kromasil 100-3.5-C4 Eluent: acetonitrile / water Eluent salt: trifluoroacetic acid After 30 preparative injections, column efficiency is impaired resulting in lower purity in extracted fractions