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The supernatant was collected, and the total GSH and GSSG levels were determined by GSH and GSSG Assay Kit (Beyotime, Jiangsu, China) following the manufacturer instruction

However, unexpectedly, K87pAcF showed the lowest stability among all the variants with the Tm value of 44.4 C, although it was predicted to be more stable than the wild type

Freezing reconstituted peptide solution is not the default recommendation for multi-use vials