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Boosting NAD + prevents human trophoblast cell dysfunction under inflammatory conditions To examine whether inflammation-mediated NAD + depletion alters human trophoblast health and function, a TNF-induced inflammatory insult was applied to the HTR8/SVneo trophoblast cell culture modelrepresentative of the invasive extravillous cytotrophoblast population, long considered a key trophoblast population contributing to the establishment of PE pathophysiology (43, 44, 45, 46)

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These syntheses employed 9-fluorenylmethoxycarbonyl-based solid-phase peptide synthesis methods, ensuring high purity and confirmed structure via liquid chromatography (LC) mass spectrometry (MS)

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