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The carbon nuclear magnetic resonance ( 13 C NMR, 100 MHz, D 2 O) signals were observed at C 25.33 (CH 2 S), 25.97 (CH 2 , C-3), 31.16 (CH 2 , C-4), 41.46 (CH 2 , C-10), 53.77 (CH, C-2), 55.61 (CH, C-7), 172.44 (C=O, C-8), 173.52 (C=O, C-5), and 174.88 (C=O, C-1 and C-11)