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Prior to transfer, stain-free gels were imaged on a Biorad Chemidoc MP Imaging System (Biorad, Hercules, CA) to confirm equal protein load, and were then wet transferred onto PVDF membranes at 100 V constant for 1 h and equal transfer was routinely confirmed by Ponceau S stain

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Rac1 normally cycles between the GTP and GDP bound states, depending on the redox state of Rac1

Our previous study demonstrated that phloretin inhibits glucose uptake and proliferation in activated CD4+ T cells and modulates the Th17/Treg balance through AMPK signaling pathway (117)