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Retrospective analysis of safety and efficacy of insulin-to-liraglutide switch in Japanese type 2 diabetes: a caution against inappropriate use in patients with reduced -cell function

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The next day, cells were carefully washed with PBS and maintained in starvation medium (HBSS (Thermo Fisher Scientific, Waltham, MA, United States) supplemented with 10 mM HEPES (Thermo Fisher Scientific, Waltham, MA, United States)) for 24 h before isolating RNA for qPCR analysis