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12.1 Asthma Evidence that pterostilbene can reducee collagen synthesis, reticular basement membrane thickness, relieve hyperplasia, and airway remodeling implicated it as an allergic asthma treatment option [206]

Dried discs were transferred to 96 deep-well blocks and treated, while shaking, with side-chain deprotection solution, consisting of 90% trifluoracetic acid (TFA), 2% dichloromethane (DCM), 5% H 2 O and 3% triisopropylsilane (TIPS) (150 L/well) for 1.5 h at room temperature (rt)

The differentiation protocol utilized was developed and optimized from the published literatures 18,19,20,21 , specifically chosen for its capacity to generate iHLOs with high CYP enzyme activity, particularly CYP3A4, which is crucial for drug metabolism and toxicology evaluations

11,20 Most studies aimed to increase GSH concentration have used glutathione precursors, such as N -acetylcysteine or sodium salts of GSH, 2 based on the assumption that oral reduced GSH is poorly absorbed and/or oxidized in the gastrointestinal tract