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Not sure the temp, whatever low is on the hot plate REHH Super Moderator #46 You need.a good hot plate if your gonna be doing this #47 It took roughly 20 minutes at low heat

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Specifically, it inhibits HIF-1-mediated aberrant activation of PDK2 while promoting LONP1-dependent selective proteasomal degradation of PDK2, thereby restoring PDH activity and enhancing ATP production [82]