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With metabolic acidosis (a reduction of the serum bicarbonate level), protons (acid) enter the cells and, to maintain electrical neutrality, potassium shifts from within the cells into the extracellular space

DNA was diluted to 100 ng l 1 in 0.4 M NaOH and 10 mM EDTA (pH 8.0) and denatured by heating at 100 C for 10 min