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It is important to note that flavonoids can exhibit both antioxidant and pro-oxidant properties, and their behavior may be influenced by factors such as test conditions, effective concentration at the site of ROS formation, stability of the flavonoid radical formed during hydrogen atom donation, lipophilicity for membrane absorption, and pH of the medium

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Amplification reactions were then implemented on a qRT-PCR system (QuantStudio 6 Flex, Life Technologies) with EnTurbo SYBR Green PCR SuperMix (EQ001, ELK Biotechnology)

In this review, we systematically summarize the latest research on the regulation of PCD by natural products in DOR-related animal and cell models, highlighting their potential roles in preserving oocyte quality and mitigating ovarian function decline