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For the measurement of total chlorophyll of leaves, a 0.5 g leaf sample was homogenized in 5 mL acetone (80% v/v), followed by centrifugation at 10,000 g for 8 min, after which the optical density was measured at 663 and 653 nm for Chl a and Chl b , respectively, using a spectrophotometer (Beckman 640 D, USA) by following the method of Arnon 34

This timing aligns IGF-1 LR3 peak plasma levels with the IGF-1 receptor upregulation triggered by the growth hormone pulse

Despite this potential synergy, the safety and effectiveness of combining these treatments remain areas that require further research

Lactic Acid Content was evaluated using the Lactic Acid (LA) HPLC Assay Kit from Solaibao, China, according to the provided instructions