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When working with an eukaryotic expression system that may contain unknown agents and factors interfering the binding of target protein, it is recommended to dilute the culture supernatant 1:1 or 1:2 with the Equilibration buffer (for example, 20 mM sodium phosphate, 0.5 M NaCl, pH 7.4)

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If you have diabetes and drink on semaglutide, check your glucose before and 23 hours after drinking

Contaminated or degraded diluents introduce variables that could cause reactions